Characterization of an Endo-Processive-Type Xyloglucanase Having a β-1,4-Glucan-Binding Module and an Endo-Type Xyloglucanase from Streptomyces avermitilis
نویسندگان
چکیده
منابع مشابه
Human Chitotriosidase Is an Endo-Processive Enzyme
Human chitotriosidase (HCHT) is involved in immune response to chitin-containing pathogens in humans. The enzyme is able to degrade chitooligosaccharides as well as crystalline chitin. The catalytic domain of HCHT is connected to the carbohydrate binding module (CBM) through a flexible hinge region. In humans, two active isoforms of HCHT are found-the full length enzyme and its truncated versio...
متن کاملthe investigation of the relationship between type a and type b personalities and quality of translation
چکیده ندارد.
Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases: biological implications for cell wall metabolism.
High-resolution, three-dimensional structures of the archetypal glycoside hydrolase family 16 (GH16) endo-xyloglucanases Tm-NXG1 and Tm-NXG2 from nasturtium (Tropaeolum majus) have been solved by x-ray crystallography. Key structural features that modulate the relative rates of substrate hydrolysis to transglycosylation in the GH16 xyloglucan-active enzymes were identified by structure-function...
متن کاملPurification and characterization of an endo-1,3-β-glucanase from Arthrobacter sp
An endo-1,3-β-glucanase from Arthrobacter sp. was purified by ammonium sulfate precipitation, anion-exchange and gel-filtration chromatographies. SDS/PAGE and gel-filtration chromatography suggested a monomer enzyme with molecular mass of 32,500. N-terminal sequence for 10 residues was APGDLLWSDE. Stable and optimum pHs were 5 to 8 and 6.5, respectively. Optimum temperature was 55°C , however, ...
متن کاملFunctional large-scale production of a novel Jonesia sp. xyloglucanase by heterologous secretion from Streptomyces lividans.
The gene encoding a novel xyloglucanase (Xeg) belonging to family 74 glycoside hydrolases was isolated from a Jonesia sp. strain through functional screening in Escherichia coli. The encoded xyloglucanase is a protein of 972 aminoacyl residues with a 23 residue aminoterminal signal peptide. Over-expression of Xeg in B. subtilis or E. coli failed. In contrast, Xeg was successfully over-expressed...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2012
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.01762-12